DIMM slot locations

Does it matter what DIMM slots are in use? dimm slot I can fill the blue DIMM slots to capacity using the RAM recommended from the respective motherboard webpages with no stability issues, but as dimm slot An ASUS TUF with so-dimm memory slot! This is a full-size ATX board that is equipped with regular DIMM slots

dimm2 slot The DIMM positions are numbered on the MB from 1 to 8 , although only 4 locations have actual slots on the motherboard, the other positions have