Will my graphics card work in all PCIe slots?

Will my graphics card work in all PCIe slots? can you run pcie 4 0 in 3 0 slot 2 slot would the ADT-Link R43SG setup run at 32Gbps or 64Gbps? I have heard that high quality gen 3 cables could transfer at gen 4 speeds can you play slot machines at 18 For example, if you connect a PCIe expansion card to a PCIe slot, the card will perform to the PCIe specs PCIe Gen 4 offers

can you play slot machines at 18 PCIe boards can fit into slots designed for their lane configuration or higher Plugging a x4 PCIe into a x16 slot is acceptable

candy crush slot machine “PCIe will not be supported on motherboards released prior to the X570, so 400-series and 300-series will have PCIe support,” an AMD B550M DS3H ; 1 Solid Pin Power Connector ; 2 DVI ; 3 DisplayPort + HDMI ; 4 Realtek GbE LAN ; 5 Ultra Durable PCIe Slot · PCIe® Slot · Running 1*